https://nova.newcastle.edu.au/vital/access/ /manager/Index en-au 5 https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:16822 2+ release from sarcoplasmic/endoplasmic reticulum Ca²⁺-ATPase (SERCA) -dependent intracellular Ca²⁺ stores; and 3) c-Kit and/or vimentin immunoreactive ICs have a role in pacemaking. Methodology/Principal Findings: Vaginal and cervical contractions were measured in vitro, as was the distribution of c-Kit and vimentin positive interstitial cells (ICs). Cervical smooth muscle was spontaneously active in estrus and metestrus but quiescent during proestrus and diestrus. Vaginal smooth muscle was normally quiescent but exhibited phasic contractions in the presence of oxytocin or the K⁺ channel blocker tetraethylammonium (TEA) chloride. Spontaneous contractions in the cervix and TEA-induced phasic contractions in the vagina persisted in the presence of cyclopiazonic acid (CPA), a blocker of the SERCA that refills intracellular SR Ca²⁺ stores, but were inhibited in low Ca²⁺ solution or in the presence of nifedipine, an inhibitor of L-type Ca²⁺channels. ICs were found in small numbers in the mouse cervix but not in the vagina. Conclusions/Significance: Cervical smooth muscle strips taken from mice in estrus, metestrus or late pregnancy were generally spontaneously active. Vaginal smooth muscle strips were normally quiescent but could be induced to exhibit phasic contractions independent on phase of the estrus cycle or late pregnancy. Spontaneous cervical or TEA-induced vaginal phasic contractions were not mediated by ICs or intracellular Ca²⁺ stores. Given that vaginal smooth muscle is normally quiescent then it is likely that increases in hormones such as oxytocin, as might occur through sexual stimulation, enhance the effectiveness of such pacemaking until phasic contractile activity emerges.]]> Wed 11 Apr 2018 13:40:08 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:18783 Wed 11 Apr 2018 12:33:36 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:37648 Tue 09 Mar 2021 14:56:52 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:37813 Tue 04 May 2021 10:12:36 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:37811 a) a well-demarcated, glazed red macule or patch at labia minora, vestibule, and/or vagina, (b) disease affects hairless skin, mucocutaneous junction, and/or nonkeratinized squamous epithelium, (c) evidence of basal layer damage, categorized as degenerative or regenerative, (d) a closely applied band-like lymphocytic infiltrate, and (e) absent subepithelial sclerosis. The clinicopathologic diagnoses of classic and hypertrophic LP each require a characteristic clinical appearance accompanied by hyperkeratosis, hypergranulosis, acanthosis, basal layer degeneration, a closely applied lymphocytic infiltrate, and absent dermal sclerosis, with hypertrophic LP showing marked epithelial abnormality compared with classic LP. Conclusions: Clinicopathological correlation yields the most reliable diagnosis of vulvar LP. Disease appearance overlaps with other physiologic, dermatologic, infectious, and neoplastic entities; a low threshold for biopsy at all morphologically distinct areas is recommended. Use of the histopathologic criteria described in this document may reduce the nondiagnostic biopsy rate for clinically diagnosed LP.]]> Tue 04 May 2021 10:05:19 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:52253 Thu 05 Oct 2023 14:14:55 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:34620 Thu 04 Apr 2019 14:26:20 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:19994 Sat 24 Mar 2018 07:50:48 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:33637 Mon 23 Sep 2019 11:03:15 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:55766 Fri 21 Jun 2024 08:26:52 AEST ]]>